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EFFECTS OF CALCIUM AND CALCIUM‐IONOPHORE ON THE OUTGROWING EPIDERMIS —POSSIBLE ACTIVATION OF EPIDERMAL PHOSPHOLIPASE A 2 —
Author(s) -
Aoyagi Takashi,
Umeda Katsuko,
Kato Naoko,
Adachi Riri,
Fukaya Toru,
Nemoto Osamu,
Kobayashi Hitoshi,
Miura Yusho
Publication year - 1983
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1983.tb01144.x
Subject(s) - ionophore , calcium , epidermis (zoology) , stimulation , chemistry , mitosis , microbiology and biotechnology , medicine , biology , endocrinology , anatomy , organic chemistry
Using an in vitro explant culture system of pig skin, the effects of calcium and calcium‐ionophore on the outgrowing epidermis were studied. The optimum concentration of calcium on the rates of epidermal outgrowth was 1.0 mM and that of mitosis was around 1.2 mM. Ionophore A23187 (Ionophore) significantly stimulated both the rates of epidermal outgrowth and the mitosis of the outgrowing epidermis. This stimulation was partially blocked by the addition of hydrocortisone. The cyclo‐oxygenation products, prostaglandins, failed to stimulate the rate of epidermal outgrowth. It appeared that the stimulatory effect of Ionophore on the outgrowing epidermis was due either to the activation of phospholipase A 2 or to the activation of the lipoxygenase pathway.