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CELL KINETIC STUDIES OF THE EFFECT OF 8‐METHOXYPSORALEN WITH LONG WAVE ULTRAVIOLET IRRADIATION ON HUMAN MELANOMA CELL LINE (SEKI, II)
Author(s) -
Shimada Shinji,
Kawashima Makoto,
Watanabe Shinichi,
Yamada Kiyoshi,
Mizoguchi Masako,
Hori Yoshiaki,
Kukita Atsushi
Publication year - 1982
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1982.tb02636.x
Subject(s) - irradiation , cell cycle , melanoma , chemistry , cell culture , cell growth , in vitro , cell , microbiology and biotechnology , biophysics , cancer research , biology , biochemistry , genetics , physics , nuclear physics
The growth inhibition and cell cycle phase distribution of a human malignant melanoma cell line (SEKI, II) treated with 8‐methoxypsoralen (8MOP) and UVA irradiation (PUVA) were investigated in vitro . A growth inhibition effect was observed in 8 methoxypsoralen (8MOP) 0.1 μ g/ml + UVA 1 J/cm 2 group, and a strong cytocidal effect was noted in the 8MOP 1 μ g/ml + UVA 3 J/cm 2 groups. Continued accumulation was observed at 24 to 72 hours in the following phases: G 2 M phase in the 8MOP 0.1 μ g/ml + UVA 1 J/cm 2 group; middle S phase in the 8MOP 0.1 μ g/ml + UVA 3 J/cm 2 group; and early S phase in the 8MOP 1 μ g/ml + UVA 1 J/cm 2 group. It appeared that 8MOP with UVA irradiation acts mainly on the S phase to block the cell cycle progression between the S and G 2 M phases. The variation of cell cycle phase distribution under the effect of 8MOP with UVA irradiation was similar to that observed with the alkylating agent, ACNU, suggesting a possible similarity of the mechanism of action between them. The reaction in the dark was vital for the onset of action of 8MOP with UVA irradiation; as is the case for DTIC with UVA irradiation (32).