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BIOCHEMICAL CHANGES AFTER THE ORAL ADMINISTRATION OF RETINOID IN THE HORNY LAYER OF PATIENTS WITH KERATINIZATION DISORDERS
Author(s) -
Yoshiike Takashi,
Negi Makoto,
Manabe Motomu,
Takamori Kenji,
Ogawa Hideoki
Publication year - 1982
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1982.tb02630.x
Subject(s) - keratin , retinoid , cystine , chemistry , gel electrophoresis , amino acid , etretinate , proline , biochemistry , chromatography , medicine , retinoic acid , cysteine , dermatology , pathology , enzyme , psoriasis , gene
Four cases with keratinization disorders were treated with aromatic retinoid (Ro 10‐9359), with resulting clinical improvement. Horny layers from these patients were obtained before and after treatment, and fractionated into keratin fibers, soluble proteins, and membraneous fractions. These were studied using SDS gel electrophoresis and amino‐acid analysis, and a comparison was made between the findings before and after retinoid administration. Membraneous fractions from diseased horny layers contained abnormal amino acid compositions, in particular, reduced half‐cystine and proline residues, which tended to become normalized after the treatment with retinoid. Normalization of keratin fibers and soluble fractions was not as obvious on SDS gel electrophoresis.