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EXPERIMENTAL STUDY OF DIFFERENCES IN HUMAN SOLE SKIN BETWEEN ONE CYCLE METHOD AND TWO CYCLE METHOD AFTER SPRAY CRYOSURGERY
Author(s) -
Ueda Keiichi,
Komori Yasushi,
Wakabayashi Shunji
Publication year - 1982
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1982.tb02609.x
Subject(s) - cryosurgery , ultrastructure , endoplasmic reticulum , vacuole , cell cycle , basal (medicine) , organelle , biophysics , chemistry , biology , anatomy , cell , microbiology and biotechnology , cytoplasm , biochemistry , medicine , endocrinology , surgery , insulin
Ultrastructural investigations were performed on human sole skin after spray cryosurgery with liquid nitrogen, using a one cycle (single freeze and thaw) method and a two cycle (double freeze and thaw) method. In the one cycle method, the epidermal cells from the basal cell layer to the spinous cell layer showed characteristic changes, i.e ., nuclear chromatin forming condensed masses, the appearance of remarkable perinuclear edematous regions, tonofibrils pushed out towards the periphery of the cells and narrow intercellular spaces with intact desmosomes. In the two cycle method, homogeneous, less dense areas were seen in the nuclei of epidermal cells from the basal cell layer to the spinous cell layer. Wide, less dense, edematous, perinuclear areas were seen in which organelles, i.e . mitochondria, endoplasmic reticulum, and vacuoles were located. Tonofibrils were pushed out to the periphery of the cells. Complete cryonecrosis was observed in cells from the basal layer to the spinous layer. Ultrastructural changes differed in degree between the one cycle freezing method and the two cycle freezing method after spray cryosurgery. It was concluded that the cryosurgical technique using the two cycle method was more effective in inducing complete cryonecrosis.