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A COMPARATIVE STUDY ON ULTRASTRUCTURE AND IMMUNOLOGICAL PROPERTIES OF HUMAN PAPILLOMA VIRUS BEFORE AND
Author(s) -
Fixation After,
Kimura Shunji,
Harada Reiko
Publication year - 1982
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1982.tb02608.x
Subject(s) - antiserum , ultrastructure , immunofluorescence , fixation (population genetics) , virus , chemistry , hela , pathology , microbiology and biotechnology , virology , biology , antigen , antibody , medicine , immunology , in vitro , biochemistry , gene
In order to clarify the effects of tissue processing on the ultrastructure and immunological properties of human papilloma virus (HPV), HPV particles were studied comparatively by negative staining and immunofluorescence (IF) technique before and after processing with various fixatives, such as formalin (F), ethanol (E), formalin and ethanol (FE), and formalin, ethanol, xylol and ethanol (FEXE). No significant ultrastructural alterations were demonstrated after processing with F, but considerable alterations were seen with E and FE. IF studies using antiserum against raw HPV revealed a remarkable decrease in specific fluorescence (SF) after processing with F or FEXE, and the disappearance of SF with E alone. However, IF studies using antiserum against formalin‐processed HPV revealed identical SF before and after processing with F or FEXE. From these findings, it is concluded that the ultrastructure of HPV may to some degree be altered by usual tissue processing, and that the reactivity of HPV to the antiserum is most seriously affected by formalin fixation of the tissue processing methods employed in preparing deparaffinized sections.