Premium
BIOCHEMICAL ANALYSES OF STRATUM CORNEUM IN HYPERKERATOSIS LENTICULARIS PERSTANS
Author(s) -
Negi Makoto,
Kubo Yukari,
Yoshiike Takashi,
Manabe Motomu,
Hirotani Tetsuya,
Ogawa Hideoki
Publication year - 1982
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1982.tb01092.x
Subject(s) - stratum corneum , keratin , chemistry , cystine , chromatography , amino acid , biochemistry , microbiology and biotechnology , biology , cysteine , enzyme , paleontology , genetics
The stratum corneum obtained from keratotic papules of hyperkeratosis lenticularis perstans was fractionated into keratin filamentous, water soluble, and membraneous fractions. SDS‐gel electrophoresis was used to examine the keratin filamentous and soluble fractions, and amino acid analysis was employed for all fractions. Electrophoresis of the keratin filamentous fraction revealed no remarkable changes in the pattern of gel bands, compared with normal controls. On the other hand, the pattern observed in the water soluble fraction showed a decrease in the number and intensity of staining bands with molecular weights within the range of 8,000–10,000. In the amino acid analysis, the half‐cystine contents of the patients' membraneous fractions were remarkably lower than those of normal membraneous fractions. Additionally, ornithine and citrulline, which are not usually found as components of protein, were determined to be moderately high in all fractions.