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DIFFERENTIAL EFFECTS OF 5‐BROMODEOXYURIDINE ON B16 MOUSE MELANOMA CELLS
Author(s) -
Sugita Yuji,
Takeuchi Takuji
Publication year - 1982
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1982.tb01076.x
Subject(s) - bromodeoxyuridine , rna , polysome , microbiology and biotechnology , immunoprecipitation , biology , polyacrylamide gel electrophoresis , gel electrophoresis , uridine , biochemistry , cell growth , enzyme , ribosome , gene
ABSTRACT The effects of 5‐bromodeoxyuridine (BrdU) on B16 mouse melanoma were studied and the following results were obtained. Melanogenesis of cells was completely inhibited by BrdU. Tyrosinase activity was reduced linearly with time by BrdU, but cytochrome c oxidase was not affected. The polysome profile from controls and that from BrdU‐treated cells resembled each other when assayed at 260 nm. However, the radioactivity in polysomes in the treated cells was about 30–40% of that in the control, when pulse labeled with radioactive uridine. Polysomal RNAs were fractionated into poly(A)‐containing and non‐poly(A)‐containing RNA with the use of poly(U)‐Sepharose, and both types of RNA were examined by polyacrylamide gel electrophoresis. No significant differences were detected between the control and the BrdU‐treated cells in either type of RNA. The effects of BrdU are specific for melanogenesis and the differences in polysomal RNAs might be too small to detect in our experiments. Immunoprecipitation of mRNAs for melanosomal proteins will be necessary to detect persuasive differences.