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ISOLATION AND CHARACTERIZATION OF THE MEMBRANEOUS FRACTION IN HUMAN STRATUM CORNEUM
Author(s) -
Manabe Motomu,
Hirotani Tetsuya,
Negi Makoto,
Hattori Michihiro,
Ogawa Hideoki
Publication year - 1981
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1981.tb02552.x
Subject(s) - chemistry , stratum corneum , trypsin , chromatography , fraction (chemistry) , pronase , amino acid , digestion (alchemy) , cystine , biochemistry , urea , enzyme , cysteine , biology , genetics
Membraneous fractions were isolated from human stratum corneum. The fractions obtained after frequent reextraction with urea and 2 ME at pH 9.0 (A), and further digestion of (A) by trypsin (B) were compared using electron microscopy and amino acid analysis. Low molecular weight proteins or amino peptides were solubilized from fraction (A) following digestion with trypsin, chymotrypsin and pronase P. The amounts of solubilized material showed a plateau after 30 minutes of digestion with trypsin. A small fraction of contaminants thought to be the fibrous and interfibrous materials were still observed in Fraction (A) by electron‐microscopy and SDS gel electrophoresis, but no remarkable contaminants were observed in Fraction (B), which was composed of trilaminar membraneous parts including the marginal band. Fraction (A) contained 43 half cystine residues per 1000 amino acid residues, whereas Fraction (B) contained 81 half cystine residues per 1000 amino acid residues.