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RETINOID INHIBITS KERATIN FORMATION OF PIG SKIN EXPLANTS
Author(s) -
Aoyagi Takashi,
Kamigaki Katsuko,
Saruta Motoji,
Iizuka Hajime,
Miura Yusho
Publication year - 1981
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1981.tb02536.x
Subject(s) - keratin , explant culture , epidermis (zoology) , in vitro , biology , microbiology and biotechnology , keratinocyte , in vivo , retinoid , anatomy , biochemistry , cell culture , genetics , retinoic acid
Using a pig explant culture system, the effects of retinoids on keratin formation in pig epidermal cells were studied. The SDS‐PAGE patterns of keratin extracted from the outgrowing epidermis in vitro were different from those of pig epidermis in vivo . The whole pig epidermis has four major keratin bands (M.W. 65,000, 60,000, 56,000 and 51,000), while the outgrowing epidermis has three major keratin bands (M.W. 63,000, 59,000 and 52,000). The SDS‐PAGE patterns of keratin proteins extracted from the outgrowing epidermis treated with Ro 10‐1670 were markedly altered. The highest molecular protein (M.W. 63,000) found in control explant was almost entirely absent in Ro 10‐1670‐treated explants and the second band (M.W. 59,000) found in control explants was split into two minor bands (M.W. 59,000 and 58,000). These results indicated that Ro 10‐1670 strongly modified keratin formation in vitro explant culture of pig skin.