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CYCLIC AMP LEVEL IN DIFFERENT LAYERS OF PSORIATIC EPIDERMIS
Author(s) -
Aoyagi Takashi,
Kamigaki Katsuko,
Miura Yusho
Publication year - 1980
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1980.tb01956.x
Subject(s) - epidermis (zoology) , phosphodiesterase , cyclase , adenylate kinase , intracellular , chemistry , biology , microbiology and biotechnology , biochemistry , enzyme , anatomy
In the present study we have determined intracellular levels of cyclic AMP in psoriatic epidermis, which had been microdissected. Involved epidermis had relatively higher cyclic‐AMP levels on a tissue weight basis than did uninvolved epidermis, but no significant differences on a DNA basis were obtained. Further microdissection of psoriatic epidermis made it possible to determine cyclic AMP levels in the upper and lower half of the epidermis, respectively. The results clearly demonstrated that the upper epidermis had significantly higher levels of cyclic AMP than the lower epidermis on a DNA basis in individual cases, but no differences between the upper and lower epidermis of involved psoriatic epidermis were obtained on a dry weight basis. It is suggested that the lower levels of cyclic AMP in the lower half of the epidermis may be due to increased activity of cyclic AMP phosphodiesterase as described by Iizuka et al., or to retarded responsiveness of adenylate cyclase to endogenous stimulators in the lower half of the epidermis where the cell surface could be more easily affected by dermal components than the upper half of the epidermis.