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AN EXPERIMENTAL MODEL OF XANTHOMA BY INTRADERMAL DEXTRAN SULFATE INJECTION
Author(s) -
Kodama Hajime,
Arakawa Kenzo,
Nagao Yo,
Tada Jyoji,
Masuda Tsutomu,
Nohara Nozomi
Publication year - 1979
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1979.tb01907.x
Subject(s) - dermis , cholesterol , chemistry , xanthoma , histiocyte , staining , infiltration (hvac) , intradermal injection , dextran , biochemistry , pathology , immunology , biology , medicine , physics , thermodynamics
Large molecular weight sodium dextran sulfate (SDS) injection into the dermis of both normolipemic rabbits and hypercholesterolemic rabbits induced infiltration of histiocytes and foam cells. Sudan III staining showed that the histiocytes and foam cells of hypercholesterolemic rabbits accumulated lipids similarly to xanthoma foam cells. The accumulated lipids increased as the injection was repeated at the same site. However, the lipid accumulation was minimal in the histiocytes of normolipemic rabbits. As determined by quantitative analysis of lipid composition in the lesions of hypercholesterolemic rabbits, cholesterol esters remarkably increased concomitantly with injection frequency. Free cholesterol accumulation was less pronounced than cholesterol ester. In normolipemic rabbit dermis, accumulation of free cholesterol was insignificant and cholesterol esters did not accumulate progressively with repeated SDS injections. Apparently the foam cells of hypercholesterolemic rabbits actively incorporated serum low density lipoproteins (LDL) and cholesterol was esterified in the foam cells. On the other hand, LDL were minimally incorporated into histiocytes in the normolipemic state. The intradermal SDS injection is a beneficial experimental model for further investigations on the mechanisms of lipid accumulation and the intracytoplasmic lipid metabolism of xanthoma foam cells.