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APPLICATION OF PROTEIN A TO THE STUDY OF PEMPHIGUS ANTIBODIES
Author(s) -
Miyagawa Sachiko,
Hashimoto Ken,
Judd Kenneth,
Lever Walter F.
Publication year - 1979
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1979.tb01906.x
Subject(s) - pemphigus , antibody , protein a , immunofluorescence , autoantibody , immunology , chemistry , microbiology and biotechnology , medicine , biology
FITC‐labeled staphylococcal protein A was used to detect pemphigus antibodies. The results showed that protein A was specific in the systems tested, although it was not as sensitive as FITC‐anti IgG in indirect immunofluorescence tests. Protein A‐adsorbent was used to isolate IgG from pemphigus serum samples. Recovery of pemphigus antibody activity was a mean of 57.1 percent, as high as that of DEAE chromatography. We consider protein A to be an alternative to monospecific anti IgG for detection of pemphigus antibodies and for purification of IgG from pemphigus sera.