Premium
ULTRASTRUCTURAL LOCALIZATION OF IMMUNOGLOBULINS IN THE DERMO‐EPIDERMAL JUNCTION OF PATIENT WITH BULLOUS PEMPHIGOID
Author(s) -
Masutani Mamoru,
Ogawa Hideoki,
Taneda Akio,
Shoji Michiko,
Miyazaki Hiroaki
Publication year - 1978
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1978.tb01056.x
Subject(s) - bullous pemphigoid , immunoelectron microscopy , basal lamina , lamina lucida , pathology , basal (medicine) , ultrastructure , chemistry , dermoepidermal junction , antibody , medicine , immunohistochemistry , immunology , dermis , insulin
Skin biopsy specimens were taken from peripheral skin of a bullous eruption of three patients with bullous pemphigoid. For immunoelectron microscopy these tissue blocks were fixed with periodate‐lysine‐paraformaldehyde. This fixation method turned out to be adequate for preservation of both tissue structure and antigenicity. The deposition of immunoglobulin and complements was observed to gradually decrease from the basal surface of the basal cells to the basal lamina. It is suggested that the antigenic substance of bullous pemphigoid is localized along the basal surface of the basal cells.