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PURINE PHOSPHORIBOSYLTRANSEERASE ACTIVITIES IN GUINEA PIG EPIDERMIS
Author(s) -
Matsuo Itsuro,
Ohkido Muneo,
Kizaki Harutoshi,
Sakurada Tomomi
Publication year - 1977
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1977.tb01029.x
Subject(s) - epidermis (zoology) , hypoxanthine , purine , purine metabolism , guinea pig , biochemistry , enzyme , substrate (aquarium) , chemistry , nucleotide , microbiology and biotechnology , biology , anatomy , endocrinology , gene , ecology
Purine phosphoribosyltransferase activities in normal and experimental hyperkeratotic epidermis of guinea pig skin were demonstrated quantitatively by a new microassay method. The ratio of HGPRTase with hypoxanthine as a substrate to APRTase activity in normal and hyperkeratotic epidermis was found to be 0.94 and 0.60, respectively. The HGPRTase and APRTase activities expressed as μmoles per gram wet weight per min. were increased in experimental hyperkeratotic epidermis and it is suggested that the salvage pathway for purine nucleotide biosynthesis is activated in experimental hyperkeratotic epidermis. The pH optimum of these enzymes and their stability in the frozen state were also demonstrated.