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ANALYSIS OF THE BINDING SITES OF PHYTOHEMAGGLUTININS AND PEMPHIGUS ANTIBODY IN THE INTERCELLULAR AREAS OF THE EPIDERMIS
Author(s) -
Nishikawa Takeji,
Harada Takashi,
Hatano Hitoshi,
Ogawa Hideoki,
Miyazaki Hiroaki
Publication year - 1976
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1976.tb00985.x
Subject(s) - pemphigus , antibody , pemphigus foliaceus , chemistry , desmoglein , antigen , microbiology and biotechnology , blocking antibody , concanavalin a , immunology , biology , biochemistry , autoantibody , in vitro
In order to characterize the pemphigus antigen(s), the binding sites of phytohemagglutinins and pemphigus antibody were studied by the combined use of fluorescein‐labelled Concanavalin A (Con A‐FITC), Phytohemagglutinin‐P‐FITC (PHA‐P‐FITC) and pemphigus antibody. Reciprocal blocking test of PHA‐P and pemphigus antibody revealed that the binding sites of PHA‐P and pemphigus antibody were independent, whereas those of Con A and pemphigus antibody were very closely related though not identical. Pemphigus skin with deposition of IgG or with presumable saturation of the binding sites with high‐titred antibody showed capability to Con A binding. Treatment of normal skin sections with Hyaluronidase or Neuraminidase did not supply any clue to the histochemical analysis of pemphigus antigen(s), since the binding sites of pemphigus antibody were destroyed in the buffer, prior to the enzyme digestion. These results suggested that the binding sites of pemphigus antibody in the intercellular areas consist, at least, of carbohydrates or glycoproteins that contain alpha‐methyl‐D‐mannoside or alpha‐methyl‐D‐glucoside.