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COMPARATIVE STUDIES ON PHOTOTOXICITY OF CHEMICALS
Author(s) -
Kobayashi Fujio,
Wada Yasushi,
Mizuno Nobuyuki
Publication year - 1974
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1974.tb01070.x
Subject(s) - phototoxicity , in vivo , erythema , in vitro , pharmacology , chemistry , dermatology , medicine , biology , biochemistry , microbiology and biotechnology
An attempt was made to establish in vitro and in vivo methods for screening phototoxic compounds. For an in vitro system, light dose‐survival curves of Escherichia coli were obtained. 8‐Methoxypsoralen (8‐MOP), bithionol (BT), 3,3′,4′,5‐tetrachlorosalicylanilide (TCSA), and 3,4′,5‐tribromosalicylanilide (TBS) showed photokilling activity against E. coli , whereas hexachlorophene (HEX), trichlorocarbanilide (TCC), and irgasan CF 3 were photoinactive. Above a certain critical dose of ultraviolet radiation, E. coli treated with TCSA or TBS exhibited some recovery from phototoxic effects. For an in vivo system, median erythema dose, rather than minimal erythema dose, was determined for guinea pig skin. 8‐MOP, BT and TCSA proved to be phototoxic in both the in vivo and in vitro systems, but in vivo phototoxicity was not shown by HEX, TCC, irgasan CF 3 , and TBS, thus demonstrating a difference in TBS phototoxicity according to the system used.