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INDUCTION OF CONTACT SENSITIVITY BY EPIDERMAL MICROSOMES DINITROPHENYLATED IN VITRO
Author(s) -
Nishioka Kiyoshi,
Nishioka Kyoko,
Aoki Toshiyuki,
Tashiro Minoru
Publication year - 1974
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1974.tb01069.x
Subject(s) - microsome , dinitrophenyl , hapten , chemistry , sensitization , in vitro , biochemistry , chromatography , biology , immunology , antibody
Epidermal microsomes were dinitrophenylated in various concentrations to obtain the optimum ratio of hapten to microsome for induction of contact sensitivity. The epidermal microsomes with 494 μg of dinitrophenyl residue produced sensitization, whereas the sensitizing ability of those with less than 114 μg of dinitrophenyl residue was incomplete or lacking. The optimum amount of dinitrophenyl residue for sensitization of all animals tested was much higher than that in the epidermal microsomes extracted from DNCB treated animals. In order to exclude the possibility of free haptens trapped in the lipid component of the microsomal membranes being responsible for the results, Sanger's method of dinitrophenylation was used. (The epidermal microsomes prepared by Sanger's method also induced contact sensitivity.) Dinitrophenylated microsomes from guinea pig livers and spleens induced contact sensitivity in some animals, but they were less effective inducers than the dinitrophenylated epidermal microsomes.