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BIOCHEMICAL AND IMMUNOLOGICAL STUDIES ON LYSOZYME FROM HAIRLESS MOUSE SKIN
Author(s) -
Ogawa Hideoki
Publication year - 1974
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1974.tb00921.x
Subject(s) - lysozyme , hairless , sephadex , egg white , chemistry , chromatography , enzyme , biochemistry , microbiology and biotechnology , biology
Lysozyme was isolated from hairless mouse skin by extraction with 0.1 N acetic acid containing 0.9 percent NaCl. Crude enzyme was purified using successive chromatographic processes on carboxymethylcellulose and Sephadex G‐50. Purified hairless mouse skin lysozyme was found to have a homogeneous electrophoretic migration pattern on polyacrylamide gel. The amino acid composition and several physico‐chemical properties of hairless mouse skin lysozyme were similar to those of human skin lysozyme, but different from those of hen egg white lysozyme. The antigenic properties of hairless mouse skin lysozyme were also closely related to those of human skin lysozyme, but not to those of hen egg white lysozyme.