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Expression of inducible nitric oxide synthase and cyclooxygenase‐2 mRNA in brain damage induced by lipopolysaccharide and intermittent hypoxia–ischemia in neonatal rats
Author(s) -
Yang Li,
Sameshima Hiroshi,
Yamaguchi Masatoshi,
Ikenoue Tsuyomu
Publication year - 2005
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/j.1341-8076.2005.00266.x
Subject(s) - nitric oxide synthase , lipopolysaccharide , hypoxia (environmental) , medicine , cyclooxygenase , brain damage , nitric oxide , ischemia , ligation , common carotid artery , endocrinology , saline , messenger rna , anesthesia , pharmacology , carotid arteries , enzyme , biology , chemistry , biochemistry , organic chemistry , oxygen , gene
Aim: The purpose of the present study was to examine the effect of lipopolysaccharide (LPS) and intermittent hypoxia–ischemia (HI) on brain damage in neonatal rats. Methods: Seven‐day‐old Wistar rats were injected with saline or LPS (1 mg/kg), and then underwent left common carotid artery ligation followed by a repetitive 8% hypoxia (2.0–4.5 min) at 10‐min intervals 10 times. The rats were divided into three groups: LPS with HI (LPS/HI, n = 46), saline with HI (HI alone, n = 42) and LPS alone ( n = 16). Seven days later, brains were assessed for neuronal damage and inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) mRNA expression. Results: Neuronal damage in the ligated side was significantly higher in LPS/HI than the other two groups ( P < 0.01). The expression of iNOS and COX‐2 mRNA was observed in the affected brain in LPS/HI, which corresponded well to histologic neuronal loss. Conclusions: LPS enhanced intermittent HI brain damage in immature animals. The expression of iNOS and COX‐2 mRNA is considered to be associated with perinatal brain injury processes.