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A character‐based approach in the Mexican cycads supports diverse multigene combinations for DNA barcoding
Author(s) -
NicolaldeMorejón Fernando,
VergaraSilva Francisco,
GonzálezAstorga Jorge,
Stevenson Dennis W.,
Vovides Andrew P.,
Sosa Victoria
Publication year - 2011
Publication title -
cladistics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.323
H-Index - 92
eISSN - 1096-0031
pISSN - 0748-3007
DOI - 10.1111/j.1096-0031.2010.00321.x
Subject(s) - dna barcoding , character (mathematics) , biology , evolutionary biology , dna , computational biology , genetics , mathematics , geometry
A DNA barcoding study was conducted to determine the optimal combination of loci needed for successful species‐level molecular identification in three extant cycad genera— Ceratozamia , Dioon , and Zamia —that occur in Mexico. Based on conclusions of a previous multigene study in representative species of all genera in the Cycadales, we tested the DNA barcoding performance of seven chloroplast coding ( matK , rpoB , rpoC1 , and rbcL ) and non‐coding ( atpF/H , psbK/I , and trnH‐psbA ) regions, plus sequences of the nuclear internal transcribed spacer. We analysed data under the assumptions of the “character attributes organization system” (CAOS), a character‐based approach in which species are identified through the presence of ‘DNA diagnostics’. In Ceratozamia , four chloroplast regions and one nuclear region were needed to achieve > 70% unique species identification. In contrast, the two‐gene combination atpF/H + psbK/I and the four‐gene combination atpF/H + psbK/I + rpoC1 + ITS2 were needed to reach 79% and 75% unique species identification in Dioon and Zamia , respectively. The combinations atpF/H + psbK/I and atpF/H + psbK/I + rpoC1 + ITS2 include loci previously considered by the international DNA barcoding community. However, none of the three combinations of potential DNA barcoding loci found to be optimal with a character‐based approach in the Mexican cycads coincides with the ‘core barcode’ of chloroplast markers ( matK + rbcL ) recently proposed for universal use in the plant kingdom.