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Cardiac remodelling of Atlantic halibut Hippoglossus hippoglossus induced by experimental anaemia with phenylhydrazine
Author(s) -
Powell M. D.,
Burke M. S.,
Dahle D.
Publication year - 2012
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2012.03327.x
Subject(s) - hippoglossus hippoglossus , halibut , biology , conceptus , medicine , zoology , andrology , fishery , fish <actinopterygii> , gestation , pregnancy , genetics
Phenylhydrazine injections (0·3 mg kg −1 , followed by a second injection of 0·1 mg kg −1 7 days later) induced a reproducible and stable anaemia in Atlantic halibut Hippoglossus hippoglossus , reducing the haematocrit and haemoglobin by 70·0 and 75·5%, respectively, over 3 weeks. There were no changes in blood electrolyte or lactate concentrations, although anaemic fish showed a 37·5 and 33·0% increase in cardiac somatic index and ventricular somatic index, respectively, compared with dimethyl sulphur oxide (DMSO) and saline vehicle controls. Changes in cardiac somatic indices did not correlate with the ratio of ventricular length:height and length:width did correlate with haematocrit and haemoglobin indicating that changes in cardiac shape may occur as a function of anaemic hypoxemia.