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Temperature dependence of cardiac sarcoplasmic reticulum Ca 2+ ‐ATPase from rainbow trout Oncorhynchus mykiss
Author(s) -
Da Silva D.,
Costa D. C. F.,
Alves C. M.,
Block B. A.,
LandeiraFernandez A. M.
Publication year - 2011
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2011.03076.x
Subject(s) - rainbow trout , biology , endoplasmic reticulum , atpase , zoology , medicine , fishery , endocrinology , fish <actinopterygii> , microbiology and biotechnology , biochemistry , enzyme
In this work, the temperature dependence of the sarco–endoplasmic reticulum Ca 2+ ‐ATPase (SERCA2) activity from rainbow trout Oncorhynchus mykiss cardiac ventricles was measured and compared with the mammalian SERCA2 isoform. The rate of ATP‐dependent Ca 2+ transport catalysed by O. mykiss vesicles was totally abolished by thapsigargin and the Ca 2+ ionophore A 23187 . At warm temperatures (25 and 30° C), the SERCA2 from O. mykiss ventricles displayed the same rate of Ca 2+ uptake. At 35° C, the activity of the O. mykiss enzyme decreased after 20 min of reaction time. The rate of Ca 2+ uptake catalysed by the mammalian SERCA2 was temperature dependent exhibiting its maximal activity at 35° C. In contrast to the rate of Ca 2+ uptake, the rate of ATP hydrolysis catalysed by O. mykiss SERCA2 was not significantly different at 25 and 35° C, but the rate of ATP hydrolysis catalysed by the rat Rattus norvegicus SERCA2 isoform at 35° C was two‐fold higher than at 25° C. At low temperatures (5 to 20° C), the rate of Ca 2+ uptake from O. mykiss SR was less temperature dependent than the R. norvegicus isoform, being able to sustain a high activity even at 5° C. The mean ± s.e . Q 10 values calculated from 25 to 35° C for ATP hydrolysis were 1·112 ± 0·026 ( n = 3) and 2·759 ± 0·240 ( n = 5) for O. mykiss and R. norvegicus , respectively. Taken together, the results show that the O . mykiss SERCA2 was not temperature dependent over the 10 to 25° C temperature interval commonly experienced by the animal in vivo . The Q 10 value of SERCA2 was significantly lower in O. mykiss than R. norvegicus which may be key for cardiac function over the wide environmental temperatures experienced in this eurythermal fish.