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Euthanization methods influence cytokine mRNA expression levels in age 0 year Oncorhynchus mykiss
Author(s) -
Bowen L.,
Kiernan J. D.,
Eder K.,
Werner I.,
Leutenegger C. M.,
Adkison M. A.,
Hedrick R. P.,
Johnson M. L.
Publication year - 2011
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2011.03034.x
Subject(s) - biology , rainbow trout , cytokine , fishery , fish <actinopterygii> , immunology
Significant differences in cytokine transcription were found between Oncorhynchus mykiss euthanized using the pharmacological agents MS‐222 v. benzocaine and also when contrasting death induced by carbon dioxide asphyxiation v. physical methods (cervical dislocation). This study highlights the need to consider the potentially confounding effect of euthanization method on gene expression data.