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Discrimination of two picarel species Spicara flexuosa and Spicara maena (Pisces: Centracanthidae) based on mitochondrial DNA sequences
Author(s) -
Imsiridou A.,
Minos G.,
Gakopoulou A.,
Katsares V.,
Karidas T.,
Katselis G.
Publication year - 2011
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2010.02858.x
Subject(s) - biology , mitochondrial dna , haplotype , 16s ribosomal rna , genetics , gene , dna sequencing , dna , zoology , evolutionary biology , genotype
A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.