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Application of single nucleotide polymorphism markers to chum salmon Oncorhynchus keta : discovery, genotyping and linkage phase resolution
Author(s) -
Garvin M. R.,
Gharrett A. J.
Publication year - 2010
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2010.02828.x
Subject(s) - oncorhynchus , biology , single nucleotide polymorphism , genotyping , snp , linkage (software) , genotype , genetics , evolutionary biology , computational biology , fishery , fish <actinopterygii> , gene
This study describes (1) the application of new methods to the discovery of informative single nucleotide polymorphism (SNP) markers in chum salmon Oncorhynchus keta , (2) a method to resolve the linkage phase of closely linked SNPs and (3) a method to inexpensively genotype them. Finally, it demonstrates that these SNPs provide information that discriminates among O. keta populations from different geographical regions of the northern Pacific Ocean. These informative markers can be used in conjunction with mixed‐stock analysis to learn about the spatial and temporal marine distributions of O. keta and the factors that influence the distributions.