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Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
Author(s) -
Díaz A. O.,
García A. M.,
Escalante A. H.,
Goldemberg A. L.
Publication year - 2010
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2010.02803.x
Subject(s) - agglutinin , biology , sialic acid , biochemistry , lectin , microbiology and biotechnology , ricinus , wheat germ agglutinin , ulex europaeus , glycoprotein
The histochemistry of glycoproteins (GP) in the mucous cells of the gills of the silverside Odontesthes bonariensis was identified with: (1) oxidizable vicinal diols; (2) sialic acid and some of their chain variants, carbon 7 ( 7 C), carbon 8 ( 8 C) or carbon 9 ( 9 C); (3) sialic acid residues without O‐acyl substitution and with O‐acyl substitution at 7 C, 8 C or 9 C; (4) carboxyl groups and (5) sulphate groups. A battery of seven biotinylated lectins allowed GPs sugar residues to be distinguished. Mucous cells showed the presence of neutral, sulphated and sialylated GPs. Dolichos biflorus agglutinin (DBA) and Glycine max agglutinin (SBA) showed strong positive staining; Arachis hypogaea agglutinin (PNA), Ricinus communis agglutinin‐I (RCA‐I) and Triticum vulgaris agglutinin (WGA) showed moderate staining, while Ulex europaeus agglutinin‐I (UEA‐I) was completely negative.