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In vitro expansion of autonomously contracting, cardiomyogenic structures from rainbow trout Oncorhynchus mykiss
Author(s) -
Grunow B.,
Ciba P.,
Rakers S.,
Klinger M.,
Anders E.,
Kruse C.
Publication year - 2010
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2009.02535.x
Subject(s) - rainbow trout , biology , trout , larva , in vitro , zoology , cell , fishery , anatomy , microbiology and biotechnology , fish <actinopterygii> , ecology , biochemistry
In primary cell preparations from larvae of rainbow trout Oncorhynchus mykiss , the formation of autonomously contracting cell aggregates was observed after 7 days. These contracting elements could be propagated and some aggregates were maintained over a period of 35 days. Electron microscopical and immunocytochemical examination revealed the presence of cardiomyocytes.