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Molecular identification of Na + –H + exchanger isoforms (NHE2) in the gills of the euryhaline teleost Fundulus heteroclitus
Author(s) -
Edwards S. L.,
Weakley J. C.,
Diamanduros A. W.,
Claiborne J. B.
Publication year - 2010
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2009.02534.x
Subject(s) - fundulus , euryhaline , biology , complementary dna , gill , killifish , gene isoform , microbiology and biotechnology , reverse transcription polymerase chain reaction , gene , gene expression , genetics , fish <actinopterygii> , fishery
In the current study, reverse‐transcription polymerase chain reaction (RT‐PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full‐length putative Na + –H + exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a protein that shows a 57% amino acid homology to rabbit NHE2. These sequences allowed data mining of available fish genome data, which revealed at least three NHE2 subtypes in some teleost species.