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PCR–RFLP analysis of mitochondrial DNA in tench Tinca tinca
Author(s) -
Lo Presti R.,
Gasco L.,
Lisa C.,
Zoccarato I.,
Di Stasio L.
Publication year - 2010
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2009.02495.x
Subject(s) - biology , restriction fragment length polymorphism , mitochondrial dna , polymerase chain reaction , genetics , haplotype , polymorphism (computer science) , population , microbiology and biotechnology , evolutionary biology , zoology , gene , genotype , demography , sociology
Polymorphism was detected at ND1, ND6, D‐loop and cyt b segments of mtDNA in 105 tench ( Tinca tinca L.), using the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique and five composite haplotypes were identified. The diversity indices and the results of the population comparisons revealed that the identified markers provide a powerful tool for further studies on this species.