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Identification of weakfish Cynoscion (Gill) in the Bay of Panama with RFLP markers
Author(s) -
VergaraChen C.,
GonzálezWangüemert M.,
Bermingham E.,
D’Croz L.
Publication year - 2009
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2009.02356.x
Subject(s) - biology , haeiii , restriction fragment length polymorphism , restriction enzyme , bay , polymerase chain reaction , panama , genetics , zoology , terminal restriction fragment length polymorphism , genetic marker , dna , ecology , gene , civil engineering , engineering
A molecular approach, polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP), was developed to identify the planktonic larvae of Cynoscion species. Species‐specific mitochondrial DNA markers were developed using three restriction endonucleases ( Dde I, Hae III and Hin fI). These markers permitted the accurate discrimination of the five Cynoscion species in the Bay of Panama.