Premium
31 P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method
Author(s) -
Thebault M. T.,
Pichavant K.,
Kervarec N.
Publication year - 2009
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2009.02351.x
Subject(s) - turbot , intracellular ph , intracellular , biology , nucleoside triphosphate , pi , nucleoside , oxygen , nuclear magnetic resonance spectroscopy , inorganic phosphate , hypoxia (environmental) , phosphate , biophysics , nuclear magnetic resonance , biochemistry , nucleotide , chemistry , physics , organic chemistry , fishery , fish <actinopterygii> , gene
A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in 31 P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure ( P O 2 ) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0· 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.