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Calibration of a hand‐held haemoglobin analyser for use on fish blood
Author(s) -
Clark T. D.,
Eliason E. J.,
Sandblom E.,
Hinch S. G.,
Farrell A. P.
Publication year - 2008
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2008.02109.x
Subject(s) - oncorhynchus , chinook wind , scomber , biology , fishery , thunnus , tuna , analyser , fish <actinopterygii> , zoology , mackerel , chemistry , chromatography
The HemoCue haemoglobin analyser consistently overestimated haemoglobin concentration ([Hb]) in the blood of all fish species (sockeye salmon Oncorhynchus nerka , Chinook salmon Oncorhynchus tshawytscha , Pacific bluefin tuna Thunnus orientalis and chub mackerel Scomber japonicus ) by 22–50% (9·9–36·0 g l −1 ) over a [Hb] range of 20–160 g l −1 . The systematic nature of the overestimation, however, allowed the formulation of an accurate calibration equation that can be used to correct values of [Hb] measured by the HemoCue in field studies.