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Development of a 37 k high‐density oligonucleotide microarray: a new tool for functional genome research in rainbow trout
Author(s) -
Salem M.,
Kenney P. B.,
Rexroad C. E.,
Yao J.
Publication year - 2008
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2008.01860.x
Subject(s) - rainbow trout , biology , microarray , computational biology , oligonucleotide , dna microarray , gene chip analysis , aquaculture , trout , genome , fishery , gene , genetics , bioinformatics , fish <actinopterygii> , gene expression
A rainbow trout high‐density oligonucleotide microarray was constructed using all tentative consensus (TC) sequences that are publicly available from all international rainbow trout Oncorhynchus mykiss genomic research projects through the Rainbow Trout Gene Index database. The new array contains 60‐mer oligonucleotide probes representing 37 394 unique TC sequences and 1417 control spots. The array (4 × 44 format) was manufactured according to the design by Agilent Technologies using the inkjet‐based SurePrint technology (design number 016320). The performance of the new microarray platform was evaluated by analysing gene expression associated with rainbow trout, vitellogenesis‐induced muscle atrophy. This microarray will open new avenues of research that will aid in the development of novel strategies for genetic improvement for economically important traits benefiting the salmonid aquaculture industries.