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RFLP and semi‐multiplex PCR‐based identification of four eel species from the south‐western Indian Ocean region
Author(s) -
Gagnaire P. A.,
Tsukamoto K.,
Aoyama J.,
Minegishi Y.,
Valade P.,
Berrebi P.
Publication year - 2007
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2007.01605.x
Subject(s) - biology , restriction fragment length polymorphism , multiplex polymerase chain reaction , mitochondrial dna , multiplex , identification (biology) , species identification , dna sequencing , polymerase chain reaction , fishery , genetics , dna , ecology , gene
To make the assignment of elvers to species more easily attainable and reliable, unpublished and recently published mitochondrial DNA sequences were used to design molecular determination approaches not based on sequencing. Two methods were proposed based on 16SrRNA single nucleotide polymorphism analysis, which improve identification of the four eel species living in the south‐western Indian Ocean region.