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Cytochemical method for staining fish basophils
Author(s) -
TavaresDias M.
Publication year - 2006
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2006.01106.x
Subject(s) - metachromasia , biology , basophil , staining , stain , toluidine , blood smear , fish <actinopterygii> , peripheral blood , cytochemistry , pathology , giemsa stain , zoology , immunology , anatomy , fishery , ultrastructure , medicine , chemistry , genetics , immunoglobulin e , malaria , antibody
The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine blue, on blood smears fixed with lead subacetate. Metachromasia was more identifiable through better preservation of the granules. The occurrence of basophils in teleost blood was confirmed as very rare and was not caused by failure to preserve the basophil granules.