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Long‐term cryopreservation of sperm of Japanese eel
Author(s) -
Tanaka S.,
Zhang H.,
Horie N.,
Yamada Y.,
Okamura A.,
Utoh T.,
Mikawa N.,
Oka H. P.,
Kurokura H.
Publication year - 2002
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2002.tb02393.x
Subject(s) - cryopreservation , biology , sperm , diluent , japanese eel , andrology , semen , anatomy , japonica , botany , fishery , embryo , chemistry , medicine , nuclear chemistry
After cryopreservation in a diluent (D#5‐D), containing 76·2 mM NaHCO3, 137·0 mM NaCl, 1·4% soya lecithin and 10% dimethyl sulphoxide, for 30–180 days, Japanese eel Anguilla japonica sperm was 61·4–70·6% motile compared with fresh sperm. Cryopreserved sperm was used for fertilizing three batches of eggs and hatchability ranged from 20·7 to 69·1%. These results indicate that D#5‐D is an useful diluent for sperm cryopreservation in Japanese eel.