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Short‐term storage of ova of common carp and tench in extenders
Author(s) -
Linhart O.,
Gela D.,
Rodina M.,
Gutierrez M. Rodriguez
Publication year - 2001
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2001.tb02366.x
Subject(s) - extender , hatching , cyprinus , biology , common carp , carp , zoology , fishery , fish <actinopterygii> , chemistry , organic chemistry , polyurethane
In a study of the effect of short‐term storage on the hatching rate of common carp Cyprinus carpio and tench Tinca tinca ova in vitro in various extenders at 21° C under aerobic conditions, the best extender for 30 min storage for common carp appeared to be Dettlaff 1. This gave the same hatching rate as controls without extender (55% v. 56%). For 60 min storage of ova, the best extenders were Dettlaff 2 (24% hatching rate) and Dettlaff 3 (30%), but hatching was significantly lower than in the control (58%). In carp ovarian artificial fluid (CAF) extender, the hatching rate of common carp ova was also high after 10 min, but decreased to 12% after 30 min. In tench, the hatching rate of ova increased after 10 min storage in Dettlaff 5 extender (44%) compared to the control (41%) without extender. However, it was significantly lower after storage in Dettlaff 1, 2, 3, 4, 5 and CAF extenders for 20, 30 and 60 min, compared to controls. Malformations (10–50%) were observed in the tench second control groups without extender after 10, 20 and 30 min storage of ova.