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Biochemical characteristics of liver and brain monoamine oxidase from pacu
Author(s) -
Salles C.,
Souza R. H. S.,
Salles J. B.,
Cunha Bastos V. L. F.,
Dias R. A.,
Cunha Bastos J.
Publication year - 2001
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2001.tb02287.x
Subject(s) - clorgyline , pacu , biology , piaractus mesopotamicus , monoamine oxidase , monoamine neurotransmitter , medicine , monoamine oxidase b , enzyme , endocrinology , biochemistry , fishery , serotonin , receptor
Monoamine oxidase (MAO) activity in the liver and brain of the pacu, Piaractus mesopotamicus was determined using a fluorescence assay with kynuramine as substrate. Apparent Michaelis constant values (20·33 μM for liver and 25·85 μM for brain) were similar in these tissues, but in terms of tissue protein MAO activity from liver was 4·5 times higher than from brain. The greater inhibitory effects of clorgyline than of deprenyl on MAO activity from liver and brain of this species suggest that pacu's MAO is a type A‐like enzyme.