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Cryopreservation of the sperm of the Japanese bitterling
Author(s) -
Ohta H.,
Kawamura K.,
Unuma T.,
Takegoshi Y.
Publication year - 2001
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2001.tb00521.x
Subject(s) - cryopreservation , extender , biology , andrology , sperm motility , glycerol , sperm , dimethylacetamide , methanol , motility , cryoprotectant , semen , diluent , liquid nitrogen , anatomy , embryo , biochemistry , chemistry , nuclear chemistry , botany , genetics , medicine , organic chemistry , solvent , polyurethane
Sperm of the Japanese bitterling Tanakia limbata that had been cryopreserved with 5 or 10% methanol plus 95 or 90% foetal bovine serum (FBS) showed higher percentage and longer duration of motility than those that had been cryopreserved with 90% FBS and 10% DMSO, glycerol, N,N‐dimethylacetamide or N, N‐dimethylformamide. Foetal bovine serum, used as extender, had some cryoprotective effects when spermatozoa were cooled either with 10% methanol or without methanol. Spermatozoa, cooled to −40° C and then immersed in liquid nitrogen, had greater post‐thaw motility than those cooled to −20, −60, or −80° C. The post‐thaw percentage of motile spermatozoa increased significantly ( P < 0·001) with decreases in the freezing rate from 60 to 5°C min −1 . These results indicate that 10% methanol plus 90% foetal bovine serum is a suitable diluent for cryopreservation of bitterling spermatozoa and that samples should be cooled to ‐40°C at a low freezing rate for effective storage.