Premium
Characteristics of sperm of captive seabass in relation to its fertilization potential
Author(s) -
Fauvel C.,
Savoye O.,
Dreanno C.,
Cosson J.,
Suquet M.
Publication year - 1999
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1999.tb00835.x
Subject(s) - biology , human fertilization , sperm , sperm motility , andrology , insemination , motility , artificial insemination , artificial fertilization , reproduction , fertility , zoology , anatomy , ecology , botany , population , microbiology and biotechnology , genetics , medicine , pregnancy , demography , sociology
Seabass Dicentrarchus labrax sperm concentration was high (up to 60 × 10 9 spz ml −1 ) but decreased significantly at the end of the reproductive season (mid‐March) in monthly sampled fish. The spermiation period may be shortened by frequent stripping. Sperm can be prediluted up to 1: 128 in non‐activating medium without loss of initial motility and motility duration. Immediately after activation by transfer to sea water, all the spermatozoa were motile for 10 s and then the number of motile cells decreased progressively but sharply to zero, so that the duration of sperm motility was very short (40 s). As a consequence, the fertility of seabass sperm decreased exponentially after 10 s following sperm activation and was zero by 1 min. The sperm requirements for optimal fertilization were c . 66 000 spermatozoa per egg. Scalingup of the experimental insemination procedure yielded better fertilization rates while conserving the individual differences due to the breeder pairs.