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Intracellular ion levels in erythrocytes and hepatocytes isolated from three teleost species
Author(s) -
Furimsky M.,
Perry S. F.,
Moon T. M.
Publication year - 1999
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1999.tb00741.x
Subject(s) - intracellular , biology , rainbow trout , hepatocyte , mole , lysis , extracellular , collagenase , biochemistry , ion transporter , in vitro , microbiology and biotechnology , membrane , enzyme , fish <actinopterygii> , fishery
Intracellular concentrations of Na + and K + were similar (∼75 mmol l −1 ) in rainbow trout Oncorhynchus mykiss hepatocytes directly following isolation by collagenase digestion, but partial recovery occurred over 6 h with K + levels increasing to 110 mmol l −1 and Na + levels decreasing to 42 mmol l −1 . Black bullhead Ameiurus melas hepatocytes exhibited higher intracellular concentrations of K + (90 mmol l −1 ) than Na + (55 mmol l −1 ) with no recovery occurring over 6 h following cell isolation. Concentrations of Na + , K + and Cl − in eel Anguilla rostrata hepatocytes were similar (∼ 55 mmol l −1 ) following isolation, with no recovery occurring over time. Erythrocytes from all species apparently did not experience an intracellular ion imbalance following isolation as indicated by high K + levels (<140 mmol l −1 ) and low Na + levels (<40 mmol l −1 ) during the entire 24‐h monitoring period. Although hepatocytes from all species exhibited an ion imbalance post‐isolation, comparison of their in vitro intracellular Na + and K + concentrations with those in plasma demonstrated that directionally correct ion gradients still exist across the cell membrane, albeit differing from those that would be found in the tissue in vivo .