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Mitochondrion‐rich cells in the embryonic yolk sac of Oreochromis mossambicus
Author(s) -
Lin H. C.,
Hwang P. P.,
Lee T. H.
Publication year - 1999
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1999.tb00643.x
Subject(s) - biology , human fertilization , yolk sac , oreochromis mossambicus , yolk , embryo , crypt , anatomy , mitochondrion , embryogenesis , microbiology and biotechnology , tilapia , andrology , fish <actinopterygii> , fishery , endocrinology , medicine
By removing a small amount of yolk, tilapia embryos were dechorionated successfully as early as 30 h after fertilization. Using DASPEI, a mitochondrion‐specific fluorescent stain, we were able to determine the first appearance of the mitochondrion rich cells on the surface of the yolk sac 26 h after fertilization ( c. 2 h after the beginning of gastrula stage). However, with scanning electron microscopy examination, no apical crypt could be found until 48 h after fertilization.