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Improved primer sequences for the mitochondrial ND1, ND3/4 and ND5/6 segments in salmonid fishes: application to RFLP analysis of Atlantic salmon
Author(s) -
Nielsen E. E.,
Hansen M. M.,
Mensberg K.L. D.
Publication year - 1998
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1998.tb00122.x
Subject(s) - biology , restriction fragment length polymorphism , mitochondrial dna , rainbow trout , primer (cosmetics) , haplotype , salmonidae , zoology , fishery , genetics , polymerase chain reaction , fish <actinopterygii> , gene , genotype , chemistry , organic chemistry
New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30 individuals from four European populations.