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Sperm structure and motility of the freshwater teleost Cottus gobio
Author(s) -
Lahnsteiner F.,
Berger B.,
Weismann T.,
Patzner R. A.
Publication year - 1997
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1997.tb01950.x
Subject(s) - motility , distilled water , biology , sperm motility , osmole , potassium , zoology , sodium , biochemistry , endocrinology , medicine , chromatography , chemistry , genetics , organic chemistry
When motility of spermatozoa of Cottos gobio was initiated with distilled water, the motility rate decreased to 0% within 1 min, and significant signs of osmotic alterations were observed at the end of the motility period. By contrast, in 50 mmol 1 −1 NaCl solution, the motility rate persisted for 120–140 min. In both distilled water and in 50 mmol 1 −1 NaCl solution, the main swimming type of spermatozoa was linear motion during the whole motility period. The initial swimming velocity (50.0 ± 2.1 μm s −1 ) measured 10 s after motility initiation was similar in both distilled water and in 50 mmol 1 −1 NaCl solution. In distilled water, the velocity decreased to <20 μm s −1 (locally motile) during the first minute of the motility phase. In 50 mmol 1 −1 NaCl solutions, it remained at a constant level during the first 60 min of the motility period, but then started to decrease to <20 μm s −1 after 120 min. When 5 mmol 1 −1 potassium cyanide, antimycin or atractyloside was added to the 50 mmol 1 −1 NaCl solution, the motility period was reduced to ≤2min. Ten millimoles per litre 2‐deoxy‐D‐glucose, malonate or a mixture of 5 mmol 1 −1 atractyloside and 5 mmol 1 −1 carnithine did not effect the duration of the motility period. This indicates that sperm energy metabolism depends mainly on respiration rate and fatty acid metabolism.