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Acid phosphatases of Esox lucius : tissue distribution and partial characterization
Author(s) -
Panara F.
Publication year - 1997
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1997.tb01665.x
Subject(s) - esox , biology , pike , acid phosphatase , sephadex , phosphatase , biochemistry , enzyme , substrate (aquarium) , hydrolysis , fish <actinopterygii> , ecology , fishery
The presence of different acid phosphatase classes was examined in organs and tissues of the fish Esox lucius . The enzymes were separated by Sephadex G‐200 column chromatography, and characterized as regards tissue distribution, molecular weight, metal ion activation, inhibition, pH optima, temperature sensitivity, rate of hydrolysis and substrate specificity. High and low molecular weight acid phosphatases as well as high and low molecular weight Zn 2+ ‐dependent acid phosphatases were present in pike organs and tissues. In addition, their biochemical properties and tissue distribution are very similar to that of respective bird and mammalian enzymes.