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Respiratory and acid‐base disturbances in rainbow trout blood during exposure to chloramine‐T under hypoxia and hyperoxia
Author(s) -
Powell M. D.,
Perry S. F.
Publication year - 1997
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1997.tb01369.x
Subject(s) - hyperoxia , hypoxia (environmental) , biology , arterial blood , chloramine , chloramine t , ventilation (architecture) , respiratory system , trout , rainbow trout , medicine , anesthesia , lung , oxygen , anatomy , chemistry , fishery , fish <actinopterygii> , chlorine , mechanical engineering , organic chemistry , engineering
Rainbow trout Oncorhynchus mykiss were exposed acutely to chloramine‐T at a therapeutic concentration (9mg l −1 ) under moderately hypoxic (water P o 2 : l00 mmHg) or hyperoxic (water P o 2 : 430mmHg) conditions and arterial blood gas tensions ( P ao 2 and P aco 2 ) and pH were monitored using an extracorporeal circulation. Hypoxia, alone, resulted in an increased ventilation frequency, a decrease in both arterial P co 2 and P o 2 and an increase in arterial pH. There was no effect of chloramine‐T exposure on the measured variables as compared with pre‐exposure (hypoxia baseline) values. Hyperoxia, alone, resulted in a decrease in ventilation frequency, an increase in arterial P co 2 and P o 2 , and a decrease in arterial pH. Chloramine‐T exposure under these conditions caused a significant increase in ventilation frequency, but no significant effect on arterial blood gases or pH as compared with the hyperoxia baseline values. Despite the increase in ventilation caused by chloramine‐T during hyperoxia, there was no reduction in P co 2 or increase in P o 2 . Although these results were of little pathological significance, this study suggests that chloramine‐T, although stimulating ventilation, was impairing the diffusion of co 2 across the gill probably by the secretion of branchial mucus and enhancing the gill boundary layer.