Premium
Specific microsatellite loci for brook charr reveal strong population subdivision on a microgeographic scale
Author(s) -
Angers B.,
Bernatchez L.,
Angers A.,
Desgroseillers L.
Publication year - 1995
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1995.tb06054.x
Subject(s) - biology , salvelinus , microsatellite , locus (genetics) , population , genetic diversity , evolutionary biology , genetics , loss of heterozygosity , population genetics , allele , zoology , trout , demography , gene , fish <actinopterygii> , fishery , sociology
We have isolated specific microsatellite loci from a partial genomic library of brook charr Salvelinus fontinalis. Their usefulness was investigated by measuring intra‐ and inter‐population genetic diversity at four loci among 20 individuals from each of five lakes located 3 to 22 km apart in La Mauricie national park (Canada). These markers were moderately to highly polymorphic. A total of five, six, 16 and 18 alleles per locus were detected, and their overall expected heterozygosity was 0.53, 0.58, 0.86 and 0.87. Strong inter‐population diversity was detected. Highly significant differences in allelic frequencies were found in all but two pairwise χ 2 permutation tests between populations at all loci. Numerous population unique alleles were observed in all five lakes. Consequently, a highly significant component of total genetic diversity was due to interpopulation variance, as exemplified by G ST values of 0.33, 0.42, 0.47 and 0.84 for each individual locus. Altogether, the results indicated that these loci should be valuable in addressing fine scale population genetics questions in brook charr. To our knowledge, they also represent the first available microsatellites developed in the genus Salvelinus.