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Cryopreservation of leucocytes of channel catfish for subsequent cytogenetic analysis
Author(s) -
Zhang Quiyang,
Tiersch T. R.
Publication year - 1995
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1995.tb06025.x
Subject(s) - cryoprotectant , cryopreservation , catfish , ictaluridae , glycerol , biology , andrology , dimethyl sulfoxide , viability assay , microbiology and biotechnology , ictalurus , biochemistry , in vitro , embryo , chemistry , fishery , fish <actinopterygii> , medicine , organic chemistry
Channel catfish leucocytes cryopreserved with glycerol or dimethyl sulphoxide (DMSO) had significantly higher ( P <0.05) viability and recovery rates than did cells cryopreserved with methanol. After 7 days of frozen storage, a 24 to 27% reduction of viability was observed for cells cryopreserved with glycerol; a 25 to 43% reduction for cells frozen with DMSO, and a 67 to 100% reduction for cells frozen with methanol. The concentration of cryoprotectants affected the viability of cryopreserved cells significantly ( p <0.05). The viability reduction was 36% for cells frozen with 5% of cryoprotectants, 30% for cells frozen with 10% of cryoprotectants, and 49% for cells frozen with 15% of cryoprotectants. The viability of cells frozen at the slower rate (‐2.7°C min −1 ) was significantly higher ( p <0.05) than that of cells frozen at the faster rate (‐45°C min −1 ). Best results were obtained for cells cryopreserved with 10% of glycerol or DMSO and frozen at the slower rate. The chromosomes prepared from cells cryopreserved using this procedure were identical to those prepared from fresh cells, and to those reported in the literature for channel Catfish.