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Cloning and sequencing of the cDNA encoding the pituitary gonadotropin II β‐subunit of yellowfin porgy ( Acanthopagrus latus )
Author(s) -
Tsai HuaiJen,
Yang LiangTung
Publication year - 1995
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1995.tb05991.x
Subject(s) - biology , complementary dna , peptide sequence , carp , silver carp , microbiology and biotechnology , catfish , protein subunit , cdna library , fishery , genetics , gene , fish <actinopterygii>
The complementary DNA (cDNA) encoding the gonadotropic hormone (GTH) II pre‐β‐subunit of yellowfin porgy ( Acanthopagrus latus ) was isolated from a pituitary gland cDNA phage library. The cDNA insert, 598 base pairs (bp), contained a 411 bp open reading frame with 35 bp and 152 bp flanking regions at the 5′‐ and 3′‐ends, respectively. The deduced amino acid sequence revealed a putative signal peptide of 24 amino acid residues and a 113 amino acid mature β‐subunit of GTH polypeptide. This pre‐β‐subunit polypeptide of the yellowfin porgy GTH II showed 86% sequence identity with that of bonito GTH II‐β 73% with killifish GTH II‐β, 63% with African catfish GTH II‐β 61% with pike eel GTH‐β, 60% with silver carp GTH‐β, 59% with chum salmon GTH 11‐β, 58% with common carp GTH‐β 57% with chinook Pacific salmon GTH‐β, and 53% with European eel GTH II‐β.