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Polymerase chain reaction‐restriction fragment length polymorphism analysis of mitochondrial rRNA genes from yellowtail rockfish
Author(s) -
McGauley K.,
Mulligan T. J.
Publication year - 1995
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1995.tb01941.x
Subject(s) - biology , rockfish , restriction fragment length polymorphism , mitochondrial dna , polymerase chain reaction , genetics , restriction fragment , terminal restriction fragment length polymorphism , gene , restriction site , restriction enzyme , ribosomal rna , restriction map , microbiology and biotechnology , zoology , fishery , base sequence , fish <actinopterygii>
A 1600 base pair region of the mitochondrial rRNA genes from 74 yellowtail rockfish, collected from three north‐east Pacific localities, was amplified via PCR. RFLP analysis yielded a total of 33 restriction sites allowing examination of 139 base pairs or 0·8% of the mtDNA molecule. Essentially no variation was detected within or among the three populations.